LOCUS       pStable-Ind-Kan-       10534 bp    DNA     circular SYN 17-JUL-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 17, 2026 from SnapGene 8.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..50
                     /label=Downstream Flanking Bases
     CDS             complement(167..1042)
                     /label=araC
                     /note="/customer_facing=True"
     regulatory      1188..1353
                     /label=araBAD promoter
                     /note="/customer_facing=True"
     CDS             1413..2558
                     /label=trfA
                     /note="/customer_facing=True"
     CDS             2932..3747
                     /label=KanR
                     /note="/customer_facing=True"
                     /note="/web_image=True"
     rep_origin      4605..5219
                     /label=oriV
                     /note="/customer_facing=True"
                     /note="/web_image=True"
     rep_origin      5295..5514
                     /label=ori2
                     /note="/customer_facing=True"
                     /note="/web_image=True"
     CDS             5605..6357
                     /label=repE
                     /note="/customer_facing=True"
     CDS             6939..8114
                     /label=sopA
                     /note="/customer_facing=True"
     CDS             8114..9082
                     /label=sopB
                     /note="/customer_facing=True"
     misc_feature    9158..9631
                     /label=sopC
                     /note="/customer_facing=True"
     misc_feature    10485..10534
                     /label=Upstream Flanking Bases
ORIGIN
        1 atcacattca gataaaaaaa atccttagct ttcgctaagg atgatttcta agcttgccgc
       61 cgcaatgctt gcataatgtg gcctgtcaaa tggacgaagc agggattctg caaaccctat
      121 gctactccgt caagccgtca attgtctgat tcgttaccaa ttattaactc aacttgacgg
      181 ctacatcatt cactttttct tcacaaccgg cacgaaactc gctcgggctg gccccggtgc
      241 attttttgaa tactcgggag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
      301 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct
      361 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgactc agacggctcg
      421 ggctcaagca aacatgctgt gcgacgctgg cgatgtcaaa attgctgtct gccaggtgat
      481 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggcggatgg aggctctcgt
      541 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctcgc
      601 tatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
      661 gctggtgcgc ttcatccggg cgaaagaaac ccgtattggc aaatatactc ggccagttaa
      721 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgtg
      781 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
      841 gtcggcagac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat
      901 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
      961 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa gctgtatccc ggcagcaggg
     1021 gatcattttg cgcttcagcc atacttttca tactcccacc attcagagaa gaaaccaatt
     1081 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctctta tcgctaaccc
     1141 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     1201 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     1261 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     1321 gctttttatc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaagg
     1381 aggaagtcgg ggcaatcccg caaggagggt gaatgaatcg gacgtttgac cggaaggcat
     1441 acaggcaaga actgatcgac gcggggtttt ccgccgagga tgccgaaacc atcgcaagcc
     1501 gcaccgtcat gcgtgcgccc cgcgaaacct tccagtccgt cggcagcatg gtccagcaag
     1561 ctacagcaaa gatcgagcgt gacagcgtgc aactggctcc accggctctg cctgcgccaa
     1621 gcgctgcagt tgagcgtagc cgtcgtctgg aacaggaggc tgcaggtttg gcgaaaagca
     1681 tgaccatcga cacgcgagga actatgacga ccaagaagcg aaaaaccgca ggcgaagatc
     1741 tggcgaaaca ggtcagcgag gcgaagcagg ccgcgttgct gaaacacacg aagcagcaga
     1801 tcaaggaaat gcagctttcc ttgttcgata ttgcgccgtg gccggacacg atgcgagcaa
     1861 tgccaaacga cacggctcgc tctgccctgt tcaccacacg caacaagaaa atcccgcgcg
     1921 aggcactgca aaacaaggtc attttccacg tcaacaagga cgtgaagatc acctacaccg
     1981 gcgtcgagct gcgggctgac gatgacgaac tggtttggct gcaagtgttg gagtacgcga
     2041 agcgtacccc tatcggcgag ccgatcacct tcacgttcta cgagctttgc caggacctgg
     2101 gctggagcat taatggccgg tattacacga aggctgagga atgcctgagc cgcctacagg
     2161 caacggcgat ggacttcacg tccgaccgcg ttggtcacct ggaaagcgtg agcctgctgc
     2221 accgcttccg cgtcctggac cgtggcaaga aaacgtcccg ttgccaggtt ctgatcgacg
     2281 aggaaatcgt agttctgttt gctggcgacc actacacgaa attcatctgg gagaagtacc
     2341 gcaagctgag tccgacggcc cgacggatgt tcgactattt cagcagtcac cgggagccgt
     2401 acccgctcaa gctggaaaca ttccgcctca tgtgcggaag cgattccacc cgcgtgaaga
     2461 agtggcgcga gcaggtcggc gaagcttgcg aagagttgcg aggcagcggc ctggttgaac
     2521 acgcctgggt caatgatgac ctggtgcatt gcaaacgcta aggccttgtg gggtcagttc
     2581 cggctggggg ttcagcagcc acccgtacga ctagcgaagg gccgtcgacc aattctcatg
     2641 tttgacagct tatcatcgaa tttctgccat tcatccgctt attatcactt attcaggcgt
     2701 agcaaccagg cgtttaaggg caccaataac tgccttaaaa aaagttcctt atcatctggc
     2761 gaatcggacc cacaagagca ctgctgatcc ttcaactcag caaaagttcg atttattcaa
     2821 caaagccacg ttgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     2881 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     2941 attcaacggg aaacgtcttg ctcccgtccg cgcttaaact ccaacatgga cgctgattta
     3001 tatgggtata aatgggctcg cgataatgtc gggcaaagtg gtgcgacaat ctatcgcttg
     3061 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     3121 gatgttacag atgagatggt ccgcctcaac tggctgacgg agtttatgcc tctcccgacc
     3181 atcaagcatt ttatccgtac tcctgatgat gcgtggttac tcaccaccgc gattcctggg
     3241 aaaacagcct tccaggtatt agaagaatat cctgatagtg gtgaaaatat tgttgatgcg
     3301 ctggccgtgt tcctgcgccg gttacatagc attcctgttt gtaattgtcc ttttaacagc
     3361 gaccgtgtat ttcgtcttgc tcaggcgcaa agtcgcatga ataacggttt ggttgatgcg
     3421 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcac
     3481 aagctcttgc cattcagtcc ggatagtgtc gtcactcatg gtgatttcag tcttgataac
     3541 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgggt cggaatcgca
     3601 gaccgttacc aggaccttgc cattctttgg aactgcctcg gtgagttttc tcctagttta
     3661 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     3721 catttgatgc tcgatgagtt tttctaaacg tctgattttc gccaaaagtt ggcccagggc
     3781 ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca
     3841 ggtatttatt cgcgataagc tcatggagcg gcgtaaccgt cgcacaggaa ggacagagaa
     3901 agcgcggatc tgggaagtga cggacagaac ggtcaggacc tggattgggg aggcggttgc
     3961 cgccgctgct gctgacggtg tgacgttctc tgttccggtc acaccacata cgttccgcca
     4021 ttcctatgcg ctgcacatgc tgtatgccgg tataccgctg aaagttctgc aaagcctgat
     4081 gggacataag tccatcagtt caacggaagt ctacacgaag gtttttgcgc tggatgtggc
     4141 tgcccggcac cgggtgcagt ttgcgatacc ggagtctgat gcggttccga tgctgaaaca
     4201 attatcctga gaataaatgc cttggccttt atatggaaat gtggaactga gtggatatgc
     4261 tgtttttgtc tgttaaacag agaagctggc tgttatccac tgagaagcga acgaaacagt
     4321 cgggaaaatc tcccattatc gtagagatcc gcattattaa tctcaggagc ctgtgtagcg
     4381 tttataggaa gtagtgttct gtcatgatgc ctgcaagcgg taacgaaaac gatttgaata
     4441 tgccttcagg aacaatagaa atcttcgtgc ggtgttacgt tgaagtggag cggattatgt
     4501 cagcaatgga cagaacaacc taatgaacac agaaccatga tgtggtctgt ccttttacag
     4561 ccagtagtgc tcgccgcagt cgagcgacag ggcgaagccc tcggctggtt gccctcgccg
     4621 ctgggctggc ggccgtctat ggccctgcaa acgcgccaga aacgccgtcg aagccgtgtg
     4681 cgagacaccg cggccggccg ccggcgttgt ggatacctcg cggaaaactt ggccctcact
     4741 gacagatgag gggcggacgt tgacacttga ggggccgact cacccggcgc ggcgttgaca
     4801 gatgaggggc aggctcgatt tcggccggcg acgtggagct ggccagcctc gcaaatcggc
     4861 gaaaacgcct gattttacgc gagtttccca cagatgatgt ggacaagcct ggggataagt
     4921 gccctgcggt attgacactt gaggggcgcg actactgaca gatgaggggc gcgatccttg
     4981 acacttgagg ggcagagtgc tgacagatga ggggcgcacc tattgacatt tgaggggctg
     5041 tccacaggca gaaaatccag catttgcaag ggtttccgcc cgtttttcgg ccaccgctaa
     5101 cctgtctttt aacctgcttt taaaccaata tttataaacc ttgtttttaa ccagggctgc
     5161 gccctgtgcg cgtgaccgcg cacgccgaag gggggtgccc ccccttctcg aaccctcccg
     5221 gtcgagtgag cgaggaagca ccagggaaca gcacttatat attctgctta cacacgatgc
     5281 ctgaaaaaac ttcccttggg gttatccact tatccacggg gatattttta taattatttt
     5341 ttttatagtt tttagatctt cttttttaga gcgccttgta ggcctttatc catgctggtt
     5401 ctagagaagg tgttgtgaca aattgccctt tcagtgtgac aaatcaccct caaatgacag
     5461 tcctgtctgt gacaaattgc ccttaaccct gtgacaaatt gccctcagaa gaagctgttt
     5521 tttcacaaag ttatccctgc ttattgactc ttttttattt agtgtgacaa tctaaaaact
     5581 tgtcacactt cacatggatc tgtcatggcg gaaacagcgg ttatcaatca caagaaacgt
     5641 aaaaatagcc cgcgaatcgt ccagagtaac gacctcactg aggcggcata tagtctctcc
     5701 cgggatcaaa aacgtatgct gtatctgttc gttgaccaga tcagaaaatc tgatggcacc
     5761 ctacaggaac atgacggtat ctgcgagatc catgttgcta aatatgctga aatattcgga
     5821 ttgacctctg cggaagccag taaggatata cggcaggcat tgaagagttt cgcggggaag
     5881 gaagtggttt tttatcgccc tgaagaggat gccggtgatg aaaaaggcta tgaatctttt
     5941 ccttggttta tcaaacgtgc gcacagtcca tccagagggc tttacagtgt acatatcaac
     6001 ccatatctca ttcccttctt tatcgggtta cagaaccggt ttacgcagtt tcggcttagt
     6061 gaaacaaaag aaatcaccaa tccgtatgcc atgcgtttat acgaatccct gtgtcagtat
     6121 cgtaagccgg atggcagtgg catcgtgtct ctgaaaatcg actggatcat agagcgttac
     6181 cagctgcctc aaagttacca gcgtatgcct gacttccgcc gccgcttcct gcaggtctgt
     6241 gttaatgaga tcaacagcag aactccaatg cgcctcagtt acattgagaa aaagaaaggc
     6301 cgccagacga ctcatatcgt attttccttc cgcgatatca cttccatgac gacaggataa
     6361 tctgagggtt atctgtcaca gatttgaggg tggttcgtca catttgttct gacctactga
     6421 gggtaatttg tcacagtttt gctgtttcct tcagcctgca tggattttct catacttttt
     6481 gaactgtaat ttttaaggaa gccaaatttg agggcagttt gtcacagttg atttccttct
     6541 ctttcccttc gtcatgtgac ttgatatcgg gggttagttc gtcatcattg atgagggttg
     6601 attatcacag tttattactc tgaattggct atccgcgtgt gtacctctac ctggagtttt
     6661 tcccacggtg gatatttctt cttgcgctga gcgtaagagc tatctgacag aacagttctt
     6721 ctttgcttcc tcgccagttc gctcgctatg ctcggttaca cggctgcggc gagcgctagt
     6781 gataataagt gactgaggta tgtgctattc ttatctcctt ttgtagtgtt gctcttattt
     6841 taaacaactt tgcggttttt tgatgacttt gcgattttgt tgttgctttg cagtaaattg
     6901 caagatttaa taaaaaaacg caaagcaatg attaaaggat gttcagaatg aaactcatgg
     6961 aaacacttaa ccagtgcata aacgctggtc atgaaatgac gaaggctatc gccattgcac
     7021 agtttaatga tgacagcccg gaagcgagga aaataacccg gcgctggaga ataggtgaag
     7081 cagcggattt agttggggtt tcttctcagg ctatcagaga tgccgagaaa gcagggcgac
     7141 taccgcaccc ggatatggaa attcgaggac gggttgagca acgtgttggt tatacaattg
     7201 aacaaattaa tcatatgcgt gatgtgtttg gtacgcgatt gcgacgtgct gaagatgtat
     7261 ttccaccggt gatcggggtt gctgcccata aaggtggcgt ttacaaaacc agtgtttctg
     7321 ttcatcttgc tcaggatctg gctctgaagg ggctacgtgt tttgctcgtg gaaggtaacg
     7381 acccccaggg aacagccagt atgtatcacg gatgggtacc agatcttcat attcatgcag
     7441 aggacactct cctgcctttc tatcttgggg aaaaggacga tgtcacttat gcaataaagc
     7501 ccacttgctg gccggggctt gacattattc cttcctgtct ggctctgcac cgtattgaaa
     7561 ctgagttaat gggcaaattt gatgaaggta aactgcccac cgatccacac ctgatgctcc
     7621 gactggccat tgaaactgtt gctcatgact atgatgtcat agttattgac agcgcgccta
     7681 acctgggtat cggcacgatt aatgtcgtat gtgctgctga tgtgctgatt gttcccacgc
     7741 ctgctgagtt gtttgactac acctccgcac tgcagttttt cgatatgctt cgtgatctgc
     7801 tcaagaacgt tgatcttaaa gggttcgagc ctgatgtacg tattttgctt accaaataca
     7861 gcaatagtaa tggctctcag tccccgtgga tggaggagca aattcgggat gcctggggaa
     7921 gcatggttct aaaaaatgtt gtacgtgaaa cggatgaagt tggtaaaggt cagatccgga
     7981 tgagaactgt ttttgaacag gccattgatc aacgttctag tactggtgcc tggagaaatg
     8041 ctctttctat ttgggaacct gtctgcaatg aaattttcga tcgtctgatt aaaccacgct
     8101 gggagattag ataatgaagc gtgcgcctgt tattccaaaa catacgctca atactcaacc
     8161 ggttgaagat actagcttaa gcacaccagc tgccccgatg gtggatagct taattgcgcg
     8221 cgtaggagta atggctcgcg gtaatgccat tactttgcct gtatgtggtc gggatgtgaa
     8281 gtttactctt gaagtgctcc ggggtgatag tgttgaaaag acctctcggg tatggagtgg
     8341 taatgaacgt gaccaggagc tgcttactga ggacgcactg gatgatctca tcccttcttt
     8401 tctactgact ggtcaacaga caccggcgtt cggtcgaaga gtatctggtg tcatagaaat
     8461 tgccgatggg agtcgccgtc gtaaagctgc tgcacttacc gaaagtgatt atcgtgttct
     8521 ggttggcgag ctggatgatg agcagatggc tgcattatcc agattgggta acgattatcg
     8581 cccaacaagt gcttatgaac gtggtcagcg ttatgcaagc cgattgcaga atgaatttgc
     8641 tggaaatatt tctgcgctgg ctgatgcgga aaatattagt cgtaagatta ttacccgctg
     8701 tatcaacacc gccaaattgc ctaaaagtgt tgttgctctt ttttctcacc ccggtgaact
     8761 atctgcccgg agtggtgatg cacttcaaaa agcctttaca gataaagagg aattacttaa
     8821 gcagcaggca tctaaccttc atgagcagaa aaaagctggg gtgatatttg aagctgaaga
     8881 agttatcact cttttaactt ctgtgcttaa aacgagttct gcaagtagaa ctagtttaag
     8941 cagtcgacat cagtttgctc ctggagcgac agtattgtat aagggcgata aaatggtgct
     9001 taacctggac aggtcccgtg ttccaactga gtgtatagag aaaattgagg ccattcttaa
     9061 ggaacttgaa aagccagcac cctgatgcga ccacgtttta gtctacgttt atctgtcttt
     9121 acttaatgtc ctttgttaca ggccagaaag cataactggc ctgaatattc tctctgggcc
     9181 cactgttcca cttgtatcgt cggtctgata atcagactgg gaccacggtc ccactcgtat
     9241 cgtcggtctg attattagtc tgggaccacg gtcccactcg tatcgtcggt ctgattatta
     9301 gtctgggacc acggtcccac tcgtatcgtc ggtctgataa tcagactggg accacggtcc
     9361 cactcgtatc gtcggtctga ttattagtct gggaccatgg tcccactcgt atcgtcggtc
     9421 tgattattag tctgggacca cggtcccact cgtatcgtcg gtctgattat tagtctggaa
     9481 ccacggtccc actcgtatcg tcggtctgat tattagtctg ggaccacggt cccactcgta
     9541 tcgtcggtct gattattagt ctgggaccac gatcccactc gtgttgtcgg tctgattatc
     9601 ggtctgggac cacggtccca cttgtattgt cgatcagact atcagcgtga gactacgatt
     9661 ccatcaatgc ctgtcaaggg caagtattga catgtcgtcg taacctgtag aacggagtaa
     9721 cctcggtgtg cggttgtatg cctgctgtgg attgctgctg tgtcctgctt atccacaaca
     9781 ttttgcgcac ggttatgtgg acaaaatacc tggttaccca ggccgtgccg gcacgttaac
     9841 cgggctgcat ccgatgcaag tgtgtcgctg tcgacgagct cgcgagctcg gacatgaggt
     9901 tgccccgtat tcagtgtcgc tgatttgtat tgtctgaagt tgcttttacg ttaagttgat
     9961 gcagatcaat taatacgata cctgcgtcat aattgattat ttgacgtggt ttgatggcct
    10021 ccacgcacgt tgtgatatgt agatgataat cattatcact ttacgggtcc tttccggtga
    10081 tccgacaggt tacggggcgg cgacctcgcg ggttttcgct atttatgaaa attttccggt
    10141 ttaaggcgtt tccgttcttc ttcgtcataa cttaatgttt ttatttaaaa taccctctga
    10201 aaagaaagga aacgacaggt gctgaaagcg agctttttgg cctctgtcgt ttcctttctc
    10261 tgtttttgtc cgtggaatga acaatggaag tccgagctca ttgctaataa cttcgtatag
    10321 catacattat acgaagttat attcgatgcg gccgcaaggg gttcgcgtca gcgggtgttg
    10381 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacg
    10441 aattcgtcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
    10501 atctgttgtt tgtcggtgaa cgctctcgga caga
//
