LOCUS       pStable-Ind-Amp-       10451 bp    DNA     circular SYN 17-JUL-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 17, 2026 from SnapGene 8.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..50
                     /label=Downstream Flanking Bases
     CDS             complement(167..1042)
                     /label=araC
                     /note="/customer_facing=True"
     regulatory      1188..1353
                     /label=araBAD promoter
                     /note="/customer_facing=True"
     CDS             1413..2558
                     /label=trfA
                     /note="/customer_facing=True"
     CDS             complement(2720..3580)
                     /label=AmpR
                     /note="/customer_facing=True"
                     /note="/web_image=True"
     regulatory      complement(3581..3685)
                     /label=AmpR promoter
                     /note="/customer_facing=True"
     rep_origin      4522..5136
                     /label=oriV
                     /note="/customer_facing=True"
                     /note="/web_image=True"
     rep_origin      5212..5431
                     /label=ori2
                     /note="/customer_facing=True"
                     /note="/web_image=True"
     CDS             5522..6274
                     /label=repE
                     /note="/customer_facing=True"
     CDS             6856..8031
                     /label=sopA
                     /note="/customer_facing=True"
     CDS             8031..8999
                     /label=sopB
                     /note="/customer_facing=True"
     misc_feature    9075..9548
                     /label=sopC
                     /note="/customer_facing=True"
     misc_feature    10402..10451
                     /label=Upstream Flanking Bases
ORIGIN
        1 atcacattca gataaaaaaa atccttagct ttcgctaagg atgatttcta agcttgccgc
       61 cgcaatgctt gcataatgtg gcctgtcaaa tggacgaagc agggattctg caaaccctat
      121 gctactccgt caagccgtca attgtctgat tcgttaccaa ttattaactc aacttgacgg
      181 ctacatcatt cactttttct tcacaaccgg cacgaaactc gctcgggctg gccccggtgc
      241 attttttgaa tactcgggag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
      301 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct
      361 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgactc agacggctcg
      421 ggctcaagca aacatgctgt gcgacgctgg cgatgtcaaa attgctgtct gccaggtgat
      481 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggcggatgg aggctctcgt
      541 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctcgc
      601 tatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
      661 gctggtgcgc ttcatccggg cgaaagaaac ccgtattggc aaatatactc ggccagttaa
      721 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgtg
      781 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
      841 gtcggcagac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat
      901 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
      961 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa gctgtatccc ggcagcaggg
     1021 gatcattttg cgcttcagcc atacttttca tactcccacc attcagagaa gaaaccaatt
     1081 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctctta tcgctaaccc
     1141 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     1201 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     1261 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     1321 gctttttatc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaagg
     1381 aggaagtcgg ggcaatcccg caaggagggt gaatgaatcg gacgtttgac cggaaggcat
     1441 acaggcaaga actgatcgac gcggggtttt ccgccgagga tgccgaaacc atcgcaagcc
     1501 gcaccgtcat gcgtgcgccc cgcgaaacct tccagtccgt cggcagcatg gtccagcaag
     1561 ctacagcaaa gatcgagcgt gacagcgtgc aactggctcc accggctctg cctgcgccaa
     1621 gcgctgcagt tgagcgtagc cgtcgtctgg aacaggaggc tgcaggtttg gcgaaaagca
     1681 tgaccatcga cacgcgagga actatgacga ccaagaagcg aaaaaccgca ggcgaagatc
     1741 tggcgaaaca ggtcagcgag gcgaagcagg ccgcgttgct gaaacacacg aagcagcaga
     1801 tcaaggaaat gcagctttcc ttgttcgata ttgcgccgtg gccggacacg atgcgagcaa
     1861 tgccaaacga cacggctcgc tctgccctgt tcaccacacg caacaagaaa atcccgcgcg
     1921 aggcactgca aaacaaggtc attttccacg tcaacaagga cgtgaagatc acctacaccg
     1981 gcgtcgagct gcgggctgac gatgacgaac tggtttggct gcaagtgttg gagtacgcga
     2041 agcgtacccc tatcggcgag ccgatcacct tcacgttcta cgagctttgc caggacctgg
     2101 gctggagcat taatggccgg tattacacga aggctgagga atgcctgagc cgcctacagg
     2161 caacggcgat ggacttcacg tccgaccgcg ttggtcacct ggaaagcgtg agcctgctgc
     2221 accgcttccg cgtcctggac cgtggcaaga aaacgtcccg ttgccaggtt ctgatcgacg
     2281 aggaaatcgt agttctgttt gctggcgacc actacacgaa attcatctgg gagaagtacc
     2341 gcaagctgag tccgacggcc cgacggatgt tcgactattt cagcagtcac cgggagccgt
     2401 acccgctcaa gctggaaaca ttccgcctca tgtgcggaag cgattccacc cgcgtgaaga
     2461 agtggcgcga gcaggtcggc gaagcttgcg aagagttgcg aggcagcggc ctggttgaac
     2521 acgcctgggt caatgatgac ctggtgcatt gcaaacgcta aggccttgtg gggtcagttc
     2581 cggctggggg ttcagcagcc acccgtacga ctagcgaagg gccgtcgacc aattctcatg
     2641 tttgacagct tatcatcgaa tttctgccat tcatccgctt attatcactt attcaggcgt
     2701 agcaaccagg cgtttaaggt taccaatgct taatcagaga ggcacctatc tcagcgatct
     2761 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     2821 agggcttacc atctggcccc agtgctgcaa tgataccgcg agatccacgc tcaccggctc
     2881 cagatttatc agcaataaac cagccagccg gaagggcaga gcgcagaagt ggtcctgcaa
     2941 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     3001 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catagtggtg tcacgctcgt
     3061 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     3121 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     3181 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatcc
     3241 catccgtaag atgcttttct gtgactgggg agtactcaac caagtcattc tgagaatagt
     3301 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     3361 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     3421 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     3481 catcttttac tttcaccagc gtttctgggt gcgcaaaaac aggaaggcaa aatgccgcaa
     3541 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actctacctt tttcaatatt
     3601 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3661 aaaataaaca aataggggtt ccgcgttggc ccagggcttc ccggtatcaa cagggacacc
     3721 aggatttatt tattctgcga agtgatcttc cgtcacaggt atttattcgc gataagctca
     3781 tggagcggcg taaccgtcgc acaggaagga cagagaaagc gcggatctgg gaagtgacgg
     3841 acagaacggt caggacctgg attggggagg cggttgccgc cgctgctgct gacggtgtga
     3901 cgttctctgt tccggtcaca ccacatacgt tccgccattc ctatgcgctg cacatgctgt
     3961 atgccggtat accgctgaaa gttctgcaaa gcctgatggg acataagtcc atcagttcaa
     4021 cggaagtcta cacgaaggtt tttgcgctgg atgtggctgc ccggcaccgg gtgcagtttg
     4081 cgataccgga gtctgatgcg gttccgatgc tgaaacaatt atcctgagaa taaatgcctt
     4141 ggcctttata tggaaatgtg gaactgagtg gatatgctgt ttttgtctgt taaacagaga
     4201 agctggctgt tatccactga gaagcgaacg aaacagtcgg gaaaatctcc cattatcgta
     4261 gagatccgca ttattaatct caggagcctg tgtagcgttt ataggaagta gtgttctgtc
     4321 atgatgcctg caagcggtaa cgaaaacgat ttgaatatgc cttcaggaac aatagaaatc
     4381 ttcgtgcggt gttacgttga agtggagcgg attatgtcag caatggacag aacaacctaa
     4441 tgaacacaga accatgatgt ggtctgtcct tttacagcca gtagtgctcg ccgcagtcga
     4501 gcgacagggc gaagccctcg gctggttgcc ctcgccgctg ggctggcggc cgtctatggc
     4561 cctgcaaacg cgccagaaac gccgtcgaag ccgtgtgcga gacaccgcgg ccggccgccg
     4621 gcgttgtgga tacctcgcgg aaaacttggc cctcactgac agatgagggg cggacgttga
     4681 cacttgaggg gccgactcac ccggcgcggc gttgacagat gaggggcagg ctcgatttcg
     4741 gccggcgacg tggagctggc cagcctcgca aatcggcgaa aacgcctgat tttacgcgag
     4801 tttcccacag atgatgtgga caagcctggg gataagtgcc ctgcggtatt gacacttgag
     4861 gggcgcgact actgacagat gaggggcgcg atccttgaca cttgaggggc agagtgctga
     4921 cagatgaggg gcgcacctat tgacatttga ggggctgtcc acaggcagaa aatccagcat
     4981 ttgcaagggt ttccgcccgt ttttcggcca ccgctaacct gtcttttaac ctgcttttaa
     5041 accaatattt ataaaccttg tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac
     5101 gccgaagggg ggtgcccccc cttctcgaac cctcccggtc gagtgagcga ggaagcacca
     5161 gggaacagca cttatatatt ctgcttacac acgatgcctg aaaaaacttc ccttggggtt
     5221 atccacttat ccacggggat atttttataa ttattttttt tatagttttt agatcttctt
     5281 ttttagagcg ccttgtaggc ctttatccat gctggttcta gagaaggtgt tgtgacaaat
     5341 tgccctttca gtgtgacaaa tcaccctcaa atgacagtcc tgtctgtgac aaattgccct
     5401 taaccctgtg acaaattgcc ctcagaagaa gctgtttttt cacaaagtta tccctgctta
     5461 ttgactcttt tttatttagt gtgacaatct aaaaacttgt cacacttcac atggatctgt
     5521 catggcggaa acagcggtta tcaatcacaa gaaacgtaaa aatagcccgc gaatcgtcca
     5581 gagtaacgac ctcactgagg cggcatatag tctctcccgg gatcaaaaac gtatgctgta
     5641 tctgttcgtt gaccagatca gaaaatctga tggcacccta caggaacatg acggtatctg
     5701 cgagatccat gttgctaaat atgctgaaat attcggattg acctctgcgg aagccagtaa
     5761 ggatatacgg caggcattga agagtttcgc ggggaaggaa gtggtttttt atcgccctga
     5821 agaggatgcc ggtgatgaaa aaggctatga atcttttcct tggtttatca aacgtgcgca
     5881 cagtccatcc agagggcttt acagtgtaca tatcaaccca tatctcattc ccttctttat
     5941 cgggttacag aaccggttta cgcagtttcg gcttagtgaa acaaaagaaa tcaccaatcc
     6001 gtatgccatg cgtttatacg aatccctgtg tcagtatcgt aagccggatg gcagtggcat
     6061 cgtgtctctg aaaatcgact ggatcataga gcgttaccag ctgcctcaaa gttaccagcg
     6121 tatgcctgac ttccgccgcc gcttcctgca ggtctgtgtt aatgagatca acagcagaac
     6181 tccaatgcgc ctcagttaca ttgagaaaaa gaaaggccgc cagacgactc atatcgtatt
     6241 ttccttccgc gatatcactt ccatgacgac aggataatct gagggttatc tgtcacagat
     6301 ttgagggtgg ttcgtcacat ttgttctgac ctactgaggg taatttgtca cagttttgct
     6361 gtttccttca gcctgcatgg attttctcat actttttgaa ctgtaatttt taaggaagcc
     6421 aaatttgagg gcagtttgtc acagttgatt tccttctctt tcccttcgtc atgtgacttg
     6481 atatcggggg ttagttcgtc atcattgatg agggttgatt atcacagttt attactctga
     6541 attggctatc cgcgtgtgta cctctacctg gagtttttcc cacggtggat atttcttctt
     6601 gcgctgagcg taagagctat ctgacagaac agttcttctt tgcttcctcg ccagttcgct
     6661 cgctatgctc ggttacacgg ctgcggcgag cgctagtgat aataagtgac tgaggtatgt
     6721 gctattctta tctccttttg tagtgttgct cttattttaa acaactttgc ggttttttga
     6781 tgactttgcg attttgttgt tgctttgcag taaattgcaa gatttaataa aaaaacgcaa
     6841 agcaatgatt aaaggatgtt cagaatgaaa ctcatggaaa cacttaacca gtgcataaac
     6901 gctggtcatg aaatgacgaa ggctatcgcc attgcacagt ttaatgatga cagcccggaa
     6961 gcgaggaaaa taacccggcg ctggagaata ggtgaagcag cggatttagt tggggtttct
     7021 tctcaggcta tcagagatgc cgagaaagca gggcgactac cgcacccgga tatggaaatt
     7081 cgaggacggg ttgagcaacg tgttggttat acaattgaac aaattaatca tatgcgtgat
     7141 gtgtttggta cgcgattgcg acgtgctgaa gatgtatttc caccggtgat cggggttgct
     7201 gcccataaag gtggcgttta caaaaccagt gtttctgttc atcttgctca ggatctggct
     7261 ctgaaggggc tacgtgtttt gctcgtggaa ggtaacgacc cccagggaac agccagtatg
     7321 tatcacggat gggtaccaga tcttcatatt catgcagagg acactctcct gcctttctat
     7381 cttggggaaa aggacgatgt cacttatgca ataaagccca cttgctggcc ggggcttgac
     7441 attattcctt cctgtctggc tctgcaccgt attgaaactg agttaatggg caaatttgat
     7501 gaaggtaaac tgcccaccga tccacacctg atgctccgac tggccattga aactgttgct
     7561 catgactatg atgtcatagt tattgacagc gcgcctaacc tgggtatcgg cacgattaat
     7621 gtcgtatgtg ctgctgatgt gctgattgtt cccacgcctg ctgagttgtt tgactacacc
     7681 tccgcactgc agtttttcga tatgcttcgt gatctgctca agaacgttga tcttaaaggg
     7741 ttcgagcctg atgtacgtat tttgcttacc aaatacagca atagtaatgg ctctcagtcc
     7801 ccgtggatgg aggagcaaat tcgggatgcc tggggaagca tggttctaaa aaatgttgta
     7861 cgtgaaacgg atgaagttgg taaaggtcag atccggatga gaactgtttt tgaacaggcc
     7921 attgatcaac gttctagtac tggtgcctgg agaaatgctc tttctatttg ggaacctgtc
     7981 tgcaatgaaa ttttcgatcg tctgattaaa ccacgctggg agattagata atgaagcgtg
     8041 cgcctgttat tccaaaacat acgctcaata ctcaaccggt tgaagatact agcttaagca
     8101 caccagctgc cccgatggtg gatagcttaa ttgcgcgcgt aggagtaatg gctcgcggta
     8161 atgccattac tttgcctgta tgtggtcggg atgtgaagtt tactcttgaa gtgctccggg
     8221 gtgatagtgt tgaaaagacc tctcgggtat ggagtggtaa tgaacgtgac caggagctgc
     8281 ttactgagga cgcactggat gatctcatcc cttcttttct actgactggt caacagacac
     8341 cggcgttcgg tcgaagagta tctggtgtca tagaaattgc cgatgggagt cgccgtcgta
     8401 aagctgctgc acttaccgaa agtgattatc gtgttctggt tggcgagctg gatgatgagc
     8461 agatggctgc attatccaga ttgggtaacg attatcgccc aacaagtgct tatgaacgtg
     8521 gtcagcgtta tgcaagccga ttgcagaatg aatttgctgg aaatatttct gcgctggctg
     8581 atgcggaaaa tattagtcgt aagattatta cccgctgtat caacaccgcc aaattgccta
     8641 aaagtgttgt tgctcttttt tctcaccccg gtgaactatc tgcccggagt ggtgatgcac
     8701 ttcaaaaagc ctttacagat aaagaggaat tacttaagca gcaggcatct aaccttcatg
     8761 agcagaaaaa agctggggtg atatttgaag ctgaagaagt tatcactctt ttaacttctg
     8821 tgcttaaaac gagttctgca agtagaacta gtttaagcag tcgacatcag tttgctcctg
     8881 gagcgacagt attgtataag ggcgataaaa tggtgcttaa cctggacagg tcccgtgttc
     8941 caactgagtg tatagagaaa attgaggcca ttcttaagga acttgaaaag ccagcaccct
     9001 gatgcgacca cgttttagtc tacgtttatc tgtctttact taatgtcctt tgttacaggc
     9061 cagaaagcat aactggcctg aatattctct ctgggcccac tgttccactt gtatcgtcgg
     9121 tctgataatc agactgggac cacggtccca ctcgtatcgt cggtctgatt attagtctgg
     9181 gaccacggtc ccactcgtat cgtcggtctg attattagtc tgggaccacg gtcccactcg
     9241 tatcgtcggt ctgataatca gactgggacc acggtcccac tcgtatcgtc ggtctgatta
     9301 ttagtctggg accatggtcc cactcgtatc gtcggtctga ttattagtct gggaccacgg
     9361 tcccactcgt atcgtcggtc tgattattag tctggaacca cggtcccact cgtatcgtcg
     9421 gtctgattat tagtctggga ccacggtccc actcgtatcg tcggtctgat tattagtctg
     9481 ggaccacgat cccactcgtg ttgtcggtct gattatcggt ctgggaccac ggtcccactt
     9541 gtattgtcga tcagactatc agcgtgagac tacgattcca tcaatgcctg tcaagggcaa
     9601 gtattgacat gtcgtcgtaa cctgtagaac ggagtaacct cggtgtgcgg ttgtatgcct
     9661 gctgtggatt gctgctgtgt cctgcttatc cacaacattt tgcgcacggt tatgtggaca
     9721 aaatacctgg ttacccaggc cgtgccggca cgttaaccgg gctgcatccg atgcaagtgt
     9781 gtcgctgtcg acgagctcgc gagctcggac atgaggttgc cccgtattca gtgtcgctga
     9841 tttgtattgt ctgaagttgc ttttacgtta agttgatgca gatcaattaa tacgatacct
     9901 gcgtcataat tgattatttg acgtggtttg atggcctcca cgcacgttgt gatatgtaga
     9961 tgataatcat tatcacttta cgggtccttt ccggtgatcc gacaggttac ggggcggcga
    10021 cctcgcgggt tttcgctatt tatgaaaatt ttccggttta aggcgtttcc gttcttcttc
    10081 gtcataactt aatgttttta tttaaaatac cctctgaaaa gaaaggaaac gacaggtgct
    10141 gaaagcgagc tttttggcct ctgtcgtttc ctttctctgt ttttgtccgt ggaatgaaca
    10201 atggaagtcc gagctcattg ctaataactt cgtatagcat acattatacg aagttatatt
    10261 cgatgcggcc gcaaggggtt cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
    10321 tatgcggcat cagagcagat tgtactgaga gtgcacgaat tcgtccaggc atcaaataaa
    10381 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
    10441 tctcggacag a
//
