LOCUS       pANSA-Kan-v1.5          3377 bp    DNA     circular SYN 17-JUL-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 17, 2026 from SnapGene 8.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..50
                     /label=Downstream Flanking Bases
     CDS             complement(296..1111)
                     /label=KanR
                     /note="/customer_facing=True"
                     /note="/web_image=True"
     rep_origin      1670..2258
                     /direction=RIGHT
                     /label=ori
                     /note="/customer_facing=True"
                     /note="/web_image=True"
     CDS             complement(2627..2817)
                     /label=rop
                     /note="/customer_facing=True"
                     /note="/web_image=True"
     misc_feature    3328..3377
                     /label=Upstream Flanking Bases
ORIGIN
        1 atcatccacg aaaagtgagc gactggattc cacgaccgac tttcctcgag catttaggac
       61 cctaccaagt caacattggt atatgaatgg agatgtttct tgtcgaactg gacagaaaaa
      121 aaaccgttat gcagtgcttt cgcactgcat aacggttttt ttttaaaaat gaagttttgg
      181 ccatttaggc cggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
      241 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctac agttattaga
      301 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
      361 atttttgaaa aagccgtttc tgtaaactag gagaaaactc accgaggcag ttccaaagaa
      421 tggcaaggtc ctggtaacgg tctgcgattc cgacccgtcc aacatcaata caacctatta
      481 atttcccctc gtcaaaaata aggttatcaa gactgaaatc accatgagtg acgacactat
      541 ccggactgaa tggcaagagc ttgtgcattt ctttccagac ttgttcaaca ggccagccat
      601 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcatgcga ctttgcgcct
      661 gagcaagacg aaatacacgg tcgctgttaa aaggacaatt acaaacagga atgctatgta
      721 accggcgcag gaacacggcc agcgcatcaa caatattttc accactatca ggatattctt
      781 ctaatacctg gaaggctgtt ttcccaggaa tcgcggtggt gagtaaccac gcatcatcag
      841 gagtacggat aaaatgcttg atggtcggga gaggcataaa ctccgtcagc cagttgaggc
      901 ggaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
      961 ctggcgcatc gggcttccca tacaagcgat agattgtcgc accactttgc ccgacattat
     1021 cgcgagccca tttataccca tataaatcag cgtccatgtt ggagtttaag cgcggacggg
     1081 agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag
     1141 cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat
     1201 tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcagc
     1261 agtgctcttg tgggtccgat tcgccagatg ataaggaact gtatttagaa aaataaacaa
     1321 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acacttaagc ctttagggtt
     1381 ttaagggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     1441 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     1501 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgta
     1561 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1621 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1681 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1741 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1801 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     1861 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     1921 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     1981 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2041 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2101 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2161 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2221 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2281 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2341 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     2401 cgaccgagcg cagcgagtca gtgagcgagg aagctatggt gcactctcag tacaatctgc
     2461 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     2521 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     2581 catccgctta cagacaagct gtgaccgact ccgggagctg catgtgtcag aggttttcac
     2641 cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgcagcg
     2701 attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta
     2761 atgtctggct tctgataaag cgggccatgt taagggcggt ttttcctgtt tggtcactga
     2821 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag
     2881 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg
     2941 taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca
     3001 gccgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg
     3061 cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa
     3121 gattccgaat accgcagttt ttggtgcatt caaagctgca ccatgaacag atcgacaatg
     3181 taacagatta acaaagattc ggcacattac taaaaaaaag gttgtttcag tgctttcgca
     3241 ctgaaacaac cttttttttt ttcgctacct tgtccaataa cgaacttcga caaccatggc
     3301 agacttaatc aggatcctga agtgtctttg caaatgtcct catagtttga acaagtccga
     3361 tcaggcttcg tcccaga
//
