LOCUS       pANSA-Amp-v1.5          3364 bp    DNA     circular SYN 17-JUL-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 17, 2026 from SnapGene 8.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..50
                     /label=Downstream Flanking Bases
     CDS             complement(296..1156)
                     /label=AmpR
                     /note="/customer_facing=True"
                     /note="/web_image=True"
     regulatory      complement(1157..1261)
                     /label=AmpR promoter
                     /note="/customer_facing=True"
     regulatory      complement(1287..1321)
                     /label=AmpR promoter
                     /note="/customer_facing=True"
     rep_origin      1657..2245
                     /direction=RIGHT
                     /label=ori
                     /note="/customer_facing=True"
                     /note="/web_image=True"
     CDS             complement(2614..2804)
                     /label=rop
                     /note="/customer_facing=True"
                     /note="/web_image=True"
     misc_feature    3315..3364
                     /label=Upstream Flanking Bases
ORIGIN
        1 atcatccacg aaaagtgagc gactggattc cacgaccgac tttcctcgag catttaggac
       61 cctaccaagt caacattggt atatgaatgg agatgtttct tgtcgaactg gacagaaaaa
      121 aaaccgttat gcagtgcttt cgcactgcat aacggttttt ttttaaaaat gaagttttgg
      181 ccatttaggc cggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
      241 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctac agttattacc
      301 aatgcttaat cagagaggca cctatctcag cgatctgtct atttcgttca tccatagttg
      361 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
      421 ctgcaatgat accgcgagat ccacgctcac cggctccaga tttatcagca ataaaccagc
      481 cagccggaag ggcagagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
      541 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
      601 ttgccattgc tacaggcata gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
      661 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
      721 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
      781 ttatggcagc actgcataat tctcttactg tcatcccatc cgtaagatgc ttttctgtga
      841 ctggggagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
      901 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
      961 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     1021 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     1081 ctgggtgcgc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     1141 aatgttgaat actcatactc tacctttttc aatattattg aagcatttat cagggttatt
     1201 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     1261 gtccgattcg ccagatgata aggaactgta tttagaaaaa taaacaaata ggggttccgc
     1321 gcacatttcc ccgaaaagtg ccacctgaca cttaagcctt tagggtttta agggaaccct
     1381 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     1441 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     1501 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtaaaa ggatctaggt
     1561 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1621 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1681 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1741 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1801 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1861 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1921 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1981 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2041 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2101 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2161 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2221 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2281 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2341 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     2401 cgagtcagtg agcgaggaag ctatggtgca ctctcagtac aatctgctct gatgccgcat
     2461 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
     2521 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     2581 acaagctgtg accgactccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     2641 acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgcagcgatt cacagatgtc
     2701 tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct
     2761 gataaagcgg gccatgttaa gggcggtttt tcctgtttgg tcactgatgc ctccgtgtaa
     2821 gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata
     2881 cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg
     2941 gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcc gaacgccagc
     3001 aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa
     3061 cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc
     3121 gcagtttttg gtgcattcaa agctgcacca tgaacagatc gacaatgtaa cagattaaca
     3181 aagattcggc acattactaa aaaaaaggtt gtttcagtgc tttcgcactg aaacaacctt
     3241 tttttttttc gctaccttgt ccaataacga acttcgacaa ccatggcaga cttaatcagg
     3301 atcctgaagt gtctttgcaa atgtcctcat agtttgaaca agtccgatca ggcttcgtcc
     3361 caga
//
