LOCUS       pAAV2-Amp-v2.1          5644 bp    DNA     circular SYN 17-JUL-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 17, 2026 from SnapGene 8.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..50
                     /label=Downstream Flanking Bases
     repeat_region   382..513
                     /label=AAV2 ITR
                     /note="/customer_facing=True"
                     /note="/web_image=True"
     CDS             1365..2156
                     /label=neo
                     /note="/customer_facing=True"
                     /note="/web_image=True"
     rep_origin      complement(2814..3402)
                     /direction=LEFT
                     /label=ori
                     /note="/customer_facing=True"
                     /note="/web_image=True"
     CDS             complement(3573..4433)
                     /label=AmpR
                     /note="/customer_facing=True"
                     /note="/web_image=True"
     regulatory      complement(4434..4538)
                     /label=AmpR promoter
                     /note="/customer_facing=True"
     repeat_region   complement(4754..4884)
                     /label=AAV2 ITR
                     /note="/customer_facing=True"
                     /note="/web_image=True"
     regulatory      4934..5313
                     /label=CMV enhancer
                     /note="/customer_facing=True"
                     /note="/web_image=True"
     regulatory      5314..5517
                     /label=CMV promoter
                     /note="/customer_facing=True"
                     /note="/web_image=True"
     misc_feature    5595..5644
                     /label=Upstream Flanking Bases
ORIGIN
        1 atccagcaca gtggcggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct
       61 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
      121 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
      181 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
      241 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
      301 aaagaaccag ctggggctta aggatcttcc tagagcatgg ctacgtagat aagtagcatg
      361 gcgggttaat cattaactac aaggaacccc tagtgatgga gttggccact ccctctctgc
      421 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
      481 gggcggcctc agtgagcgag cgagcgcgca gctagggggt atccccacgc gccctgtagc
      541 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
      601 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
      661 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
      721 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
      781 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
      841 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
      901 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
      961 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     1021 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     1081 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     1141 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     1201 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     1261 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     1321 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     1381 gattgcacgc aggatctccg gccgcttggg tggagaggct attcggctat gactgggcac
     1441 aacagacaat cggctgctct gatgccgccg tgttccggct gagtgcgcag gggcgcccgg
     1501 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     1561 ggctaagttg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     1621 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcctctc
     1681 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     1741 ttgatccggc tacttgccca ttcgaccacc aagcgaaaca tcgcatcgaa cgagcacgta
     1801 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaggagcat caggggctcg
     1861 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     1921 tgacccatgg cgatgcatgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     1981 tcatcgactg tggccggctg ggtgtcgcgg accgctatca ggacatagcg ttggctaccc
     2041 gtgatattgc tgaggagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     2101 tcgccgctcc cgatagtcag cgcatcgcct tctatcgcct tcttgacgag ttcttctaag
     2161 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctggcat cacgagattt
     2221 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     2281 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     2341 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     2401 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     2461 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     2521 gtgaaaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     2581 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttgcgct
     2641 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2701 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2761 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2821 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2881 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2941 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3001 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     3061 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     3121 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3181 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3241 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3301 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3361 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3421 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     3481 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     3541 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag ggaggcacct
     3601 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     3661 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagatcca
     3721 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc ggagcgcaga
     3781 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     3841 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     3901 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     3961 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     4021 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     4081 cttactgtca tgccatccgt aagatgcttt tctgtgactg gggagtactc aaccaagtca
     4141 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     4201 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     4261 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     4321 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     4381 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     4441 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     4501 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     4561 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     4621 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     4681 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     4741 agaatctgct taggctgcgc gctcgctcgc tcactgaggc cgcccgggca aagcccgggc
     4801 gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg
     4861 ccaactccat cactaggggt tcctgttagg cgttttgcgc tgcttcgcga tgtacgggcc
     4921 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     4981 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     5041 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     5101 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     5161 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     5221 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     5281 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     5341 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     5401 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     5461 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
     5521 tggctaacta gagaacccac tgcttactgg cttatcgaaa tgacacccaa gctggctagc
     5581 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
     5641 agat
//
